Human HDAC6, GenBank Accession No. BC069243, full length with N-terminal GST-tag, MW= 159 kDa, expressed in baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: >=450 pmol/min/ug.
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
50 mM Tris-HCl, pH 7.5, 500 mM NaCl, 50 mM KCl, 2 mM EDTA, 10% glycerol, and 2 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Purification
Protein was purified by affinity chromatography and gel filtration.
Notes
25 mM Tris/HCl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mMMgCl2, and 0.1 mg/ml BSA, 20 uMBPS HDAC substrate 3 (Catalog#50037), and HDAC6 (3.1 - 200 ng).Incubation condition: 30 min at 37°Cfollowed by developing for 15 min atroom temperature.