Mouse HDAC6, also known as histone deacetylase 6, GenBank Accession No. NM_010413, a.a. 2-1149 (end) with N-terminal GST-Tag, expressed in baculovirus expression system. MW = 152 kDa.
Host cell line: Sf9 cells
Immunogen Region
2-1149 (end)
Tag
N-terminal GST tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol
Species
Mouse
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20uM BPS HDAC substrate 3 (Catalog #50037), and Mouse HDAC6 (3.1 - 200 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.
Avoid freeze/thaw cycles.
GenBank
NM_010413
Mw(kda)
152 kDa
Synonyms
histone deacetylase 6, HDAC 6, HDAC-6
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Reference
1. Rikimaru, T. et al., Oncology 72(1-2), 69-74 (2007).2. Ishihama, K. et al., J Clin Pathol 60(11), 1205-10 (2007).