Human HDAC6, a.a. 1-1215, N-terminal GST tag, GenBank Accession No. NM_006044, expressed in a HEK293 expression system. MW = 161 kDa.
Specific activity: 300 pmol/min/ug
Immunogen Region
1-1215
Tag
N-terminal GST tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 3mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate 3 (Catalog #50037), and HDAC6 (3.2 - 200ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.
Avoid freeze/thaw cycles.
GenBank
NM_006044
Mw(kda)
161 kDa
Uniprot
Q9UBN7
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 12 months at -80°C
Reference
1. Li S. et al., Neurology. 41(2), 112-6 (2010).2. Strausberg, R.L. et al., Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002).