Human HDAC5 (GenBank Accession No. NM_005474), a.a. 1-1122 (end) with N-terminal GST-tag and C-terminal His-tag, expressed in a HEK293 expression system. MW=149 kDa.
Immunogen Region
1-1122
Tag
N-terminal GST-tag, C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16mM Glutathione, 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate 1 (Catalog #50040), and HDAC5 (0.3125 - 20 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_005474
Mw(kda)
149 kDa
Synonyms
histone deacetylase 5, HDAC-5
Uniprot
Q9UQL6
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Purity
>=55%
Storage
At least 6 months at -80°C.
Reference
1. Spiegelberg, B.D. and Hamm, H.E. (2005) J. Biol. Chem. 280, 41769-417762. Waltregny et al., (2004) J. Neural Transm. 48, 273-290.