Human HDAC11 (full length) (GenBank Accession No. NM_024827) untagged, MW= 39.6 kDa, expressed in baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: >= 1500 pmol/min/ug
Immunogen Region
full length
Tag
none
Formulation
50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 50 mM KCl, 2 mM EDTA, 3 mM DTT, 0.5% Triton-X-100 and 10% Glycerol.
Species
human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
25 mM Tris/HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.05% Triton X-100, 0.1 mg/ml BSA, 20 uM BPS HDAC substrate 1 (Catalog #50040), and HDAC11 (0.4 - 25 ng). Incubation condition: 30 min at 37°C followed by developing for 15 min at room temperature.