Human HDAC10 (a.a. 2-631) (GenBank Accession No. NM_032019) with N-terminal FLAG-tag, MW= 67 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: >=3.6 pmol/min/ug.
Immunogen Region
2-631
Tag
N-terminal FLAG-tag
Formulation
45 mM Tris-HCl, pH 8.0, 124mM NaCl, 2.4 mM KCl, 0.05% Tween-20 and10% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
25 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC substrate 3 (Catalog #50037), and 40 ng/ul HDAC10. Incubation condition: 30 min. at 37°C.