Fusion protein corresponding to theserine protease NS3 (a.a. 3-181) andcofactor NS4A (a.a. 21-32) from HepatitisC virus genotype 1a (HCV1a), GenBankAccession No. NC_004102, with Arg-to-Lys mutation on a.a. 155, and N-terminalFLAG-His tag, MW = 22.4 kDa,expressed in an E. coli expressionsystem.
Immunogen Region
21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3
Tag
N-terminal FLAG-His-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, and 300 mM imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling