Fusion protein corresponding to the serine protease NS3 (a.a. 3-181) and cofactor NS4A (a.a. 21-32) from Hepatitis C virus genotype 1a (GenBank Accession No. NC_004102) with Asp-to-Val mutation on a.a. 168, an N-terminal FLAG-His tag, and a 4 a.a. linker, MW = 22.4 kDa, expressed in an E. coli expression system.
Immunogen Region
21-32 of NS4A, linker (4 a.a.) and a.a. 3-181 of NS3
Tag
N-terminal FLAG-His-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 250 mM imidazole.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling