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Home / Proteins & Peptide

H1N1 (A/California/04/2009), Hemagglutinin

SKU : BHP18500342

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iXCells Biotechnologies

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Mfr.No.	IX03181, IX03181-10, IX03181-100
Description	Influenza hemagglutinin (HA) is a glycoprotein found on the surface of the influenzavirus. It is responsible for binding the virus to cells with sialic acid on their membranes, such as cells in the upper respiratory tract or erythrocytes. It is also responsible for the fusion of the viral envelope with the endosome membrane after the pH has been reduced. The name "hemagglutinin" comes from the protein's ability to cause red blood cells (erythrocytes) to clump together in vitro. HA has two functions. First, it allows the recognition of target vertebrate cells, accomplished through binding to these cells' sialic acid-containing receptors. Second, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. H1N1 is a subtype of influenza virus A and the most common cause of influenza in humans. Recombinant Influenza A H1N1 (A/California/04/2009(H1N1)) Hemagglutinin with his-tag produced in Sf9 Cell is a single, glycosylated polypeptide chain containing 520 amino acids. A fully biologically active molecule, HA-H1N1 has a molecular mass of ~66 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Reconstitution	Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower.
Formulation	Lyophilized in 20 mM PB buffer (pH 7.4), 300 mM NaCl, 5% mannitol, 5% trehalose.
Molecular Weight	~66 kDa, observed by reducing SDS-PAGE.
Source	Sf9 insect cells
Host	Hemagglutinin
Accession Number	ACP41105.1
Endotoxin Level	1 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	This recombinant protein has not been tested.

Synonyms	HA
Sequence	MKAILVVLLY TFATANADTL CIGYHANNST DTVDTVLEKNVTVTHSVNLL EDKHNGKLCK LRGVAPLHLG KCNIAGWILGNPECESLSTA SSWSYIVETP SSDNGTCYPG DFIDYEELREQLSSVSSFER FEIFPKTSSW PNHDSNKGVT AACPHAGAKSFYKNLIWLVK KGNSYPKLSK SYINDKGKEV LVLWGIHHPSTSADQQSLYQ NADTYVFVGS SRYSKKFKPE IAIRPKVRDQEGRMNYYWTL VEPGDKITFE ATGNLVVPRY AFAMERNAGSGIIISDTPVH DCNTTCQTPK GAINTSLPFQ NIHPITIGKCPKYVKSTKLR LATGLRNIPS IQSRGLFGAI AGFIEGGWTGMVDGWYGYHH QNEQGSGYAA DLKSTQNAID EITNKVNSVIEKMNTQFTAV GKEFNHLEKR IENLNKKVDD GFLDIWTYNAELLVLLENER TLDYHDSNVK NLYEKVRSQL KNNAKEIGNGCFEFYHKCDN TCMESVKNGT YDYPKYSEEA KLNREEIDGVKLESTRIYQH HHHHHHH

Purity	95% by SDS-PAGE and HPLC analyses.
Storage	Lyophilized recombinant Influenza A H1N1 (A/California/04/2009(H1N1)) Hemagglutinin remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, HA-H1N1 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500342-1	IX03181-10	10ug	$49		Add to Cart
BHP18500342-2	IX03181-100	100ug	$245		Add to Cart

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