Human glycogen synthase kinase 3 alpha (GSK3alpha), also known as GSK3a and GSK3alpha, GenBank Accession No. NM_019884, a.a. 2-483(end), with N-terminal GST-tag and C-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system, MW=78 kDa.
Specific activity: 109 pmol/min/ug
Immunogen Region
2-483(end)
Tag
N-terminal GST-tag, C-terminal His-tag
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl,0.04% Tween20, 3 mM DTT, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed according to the GSK3alpha Assay Kit (Biohippo, #79698) with GSK3a titrated from 200 ng/rxn- 0 ng/rxn.
Avoid freeze/thaw cycles.
GenBank
NM_019884
Mw(kda)
78 kDa
Synonyms
Human glycogen synthase kinase 3 alpha, GSK3a, GSK3alpha
Uniprot
P49840
Shipping Temperature
-80°C (dry ice)
Storage
At least 6 months at -80°C.
Reference
1. Meijer, L. et al., Trends Pharmacol Sci. 25 (9), 471-480 (2004). 2. Patel, S. et al., Biochem Soc. Trans. 32(pt5): 803-808 (2004).