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Home / Proteins & Peptide

GSK3beta, GST-tag, His-tag

SKU : BHP18200128

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BPS Bioscience

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Mfr.No.	40007
Description	Human GSK3beta GenBank Accession No. NM_002093), full length, with N-terminal GST-tag and C-terminal His-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: 391 pmol/min/ug
Immunogen Region	full length
Tag	N-terminal GST-tag, C-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reactions include 50 ul volumes of 1x Kinase buffer (40 mM Tris pH 7.4, 20 mM MgCl₂, 0.1 mg/mL BSA) along with 20 uM ATP, 0.2 mg/mL GSKtidesubstrate, 3mM DTT, and GSTGSK3. After 45 min incubation at30°C, 50uL of the Kinase-Glo reagent solution was added and luminescence from remaining ATP was measured. Avoid freeze/thaw cycles.
GenBank	NM_002093
Mw(kda)	73 kDa

Synonyms	GSK3b, glycogen synthase kinase 3 beta, GSK3beta
Uniprot	P49841

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Sutherland C. et al. Biochem J. 1993 Nov 15;296 (Pt 1):15-9. 2. Sperber BR et al. Neurosci Lett. 1995 Sep 8;197(2):149-53.Application Reference(s): 1.Glycogen synthase kinase-3beta inhibition in the medial prefrontal cortex mediates paradoxical amphetamine action in a mouse model of ADHD (2015) 2.Induction of Gsk3beta-beta-TrCP interaction is required for late phase stabilization of beta-catenin in canonical Wnt signaling (2014) 3. Leucine-rich repeat kinase 2 regulates tau phosphorylation through direct activation of glycogen synthase kinase-3beta (2014)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200128	40007	10 ug	$401.50		Add to Cart

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