Human GSK3alpha (alpha) GenBank Accession No. BC027984), full length, with N-terminal GST-tag, MW=81.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 181 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, 2 uM substrate (Ser/Thr 9, Z-Lyte Kinase Assay Kit, Invitrogen #PV3324), and GSK3alpha at 37°C for 1 hour.