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Home / Proteins & Peptide

GM-CSF (Sargramostim), Human (P. pastoris-expressed)

SKU : BHP18500027

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iXCells Biotechnologies

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Mfr.No.	IX02693, IX02693-1, IX02693-10, IX02693-50
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Description	Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines (1). A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds(2,3), glycosylated and non-glycosylated hGM-CSFs show similar biological activities(4). Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells(5).Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in P. pastoris a glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 26-32kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Formulation	Lyophilized after extensive dialysis against 10 mM PB, pH7.0.
Molecular Weight	26-32kDa, observed by SDS-PAGE.
Source	P. pastoris
Host	Human
Endotoxin Level	1.0EU/ug, determined by LAL method.
Bioactivity	ED500.2ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of 5x 106 units/mg.

Synonyms	Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897
Sequence	MAPARSPSPS TQPWEHVNAI QEALRLLNLS RDTAAEMNETVEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTMMASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPFDCWEPVQE

Purity	95 % as analyzed by SDS-PAGE.
Storage	Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500027-1	IX02693-1	1mg	$2621		Add to Cart
BHP18500027-2	IX02693-10	10ug	$107		Add to Cart
BHP18500027-3	IX02693-50	50ug	$344		Add to Cart

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