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Home / Proteins & Peptide

GLS1, His-tag

SKU : BHP18200581

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Mfr.No.	71102
Description	Human glutaminase (GLS), also known as glutaminase transcript variant 1 (GLS1), GAC, GAM and KGA, GenBank Accession No. NM_014905, a.a. 17-669(end), with N-terminal His-tag, MW 72.5 kDa, expressed in an E. coli cell expression system. Specific activity: >= 25 nmol/min/ug
Background	GLS1 is a phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. GLS1 is primarily expressed in the brain and kidney where it catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. It plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Immunogen Region	17-669(end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The reaction mixture contained 100 mM Tris, pH 8.6, 150 mM K₂HPO₄, 25 mM L-glutamine, 2 mM NAD+, 4 units of GDH, and various amounts of GLS1 in a volume of 200 l. Reactions were initiated with the addition of GLS1, and the increase in NADH absorbance at 340 nm was continuously monitored for 7 minutes at 37°C. Molar extinction coefficient of NADH is 6,220 M^-1^cm-1. Avoid freeze/thaw cycles.
GenBank	NM_014905
Mw(kda)	72.5 kDa

Synonyms	glutaminase, glutaminase transcript variant 1, GLS1, GAC, GAM, KGA, GLS
Uniprot	O94925

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Van den Heuvel, A.P., et al., Cancer Biol. Ther. 2012;13(12):1185-1194. 2. Thangavelu, K., et al., Proc. Natl. Acad. Sci. USA 2012;109(2):7705-7710.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200581	71102	20 ug	$610.50		Add to Cart

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