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Home / Proteins & Peptide

Galactokinase, His-tag

SKU : BHP18201507

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Mfr.No.	50275-2
Immunogen Region	2 - 399 (end)
Tag	N-terminal His-tag
Description	Human galactokinase (GenBank Accession No. NM_000154), full length with N-terminal His-tag, MW=42 kDa, expressed in an E. coli expression system. Specific activity: >=176 pmoles/min/ug
Formulation	45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol, 225 mM imidazole, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	A 50 ul Galactokinase reaction isconducted in a buffer containing 20 mMTris (pH 7.4), 5 mM MgCl₂, 50 mM NaCl,50 uM ATP, 1 mM DTT and 1 mMsubstrate D-Galactose at roomtemperature for 1h. ATP reduction isdetected using Kinase-Glo LuminescentKinase Assay Platform. Avoid freeze/thaw cycles.
GenBank	NM_000154
Mw(kda)	42 kDa

Synonyms	GALK1
Uniprot	P51570

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Thoden J.B. et al., J. Biol. Chem. 280 (10), 9662-9670 (2005). 2. Holden H.M. et al., Cell. Mol. Life Sci. 61 (19-20), 2471-2484 (2004).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201507	50275-2	200 ug	$555.50		Add to Cart

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