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Home / Proteins & Peptide

gAcrp30/Adipolean, Human

SKU : BHP18500258

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iXCells Biotechnologies

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Mfr.No.	IX03091, IX03091-1, IX03091-10, IX03091-50
Reconstitution	Reconstituted in ddH2O at 100 ug/mL.
Description	gAcrp30/Adipolean is the globular head domain of Adipocyte complement-related protein of 30 kDa (Acrp30), a cytokine expressed in adipocytes. The name of Acrp30 is bases on its closest homolog, complement factor c1q, and the globular domain of Acrp30 has an unexpected homolog with the Tumor Necrosis Factor (TNF) family of cytokines. Acrp30 is the isoform of adiponectin, and shares the two receptors with adiponectin: adipoR1 expressed in skeletal muscle, and adipoR2 expressed in liver. The expression level of Acrp30 in adipocytes is negatively correlated with body weight, and is lower in obese mouse than normal mouse. The globular domain of Acrp30 induces free fatty acid oxidation in muscle and weight reduction in mouse, therefore shows potential pharmacological effects in obesity. Recombinant human gAcrp30/Adipolean (rhgAcrp30) produced in E. coli is a single non-glycosylated polypeptide chain containing 144 amino acids. A fully biologically active molecule, rhgAcrp30 has a molecular mass of 16.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques.
Formulation	Lyophilized after extensive dialysis against PBS.
Molecular Weight	16.6 kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Human
Accession Number	Q15848
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 10 ug/mL, measured by a cell growth inhibition assay using M1 cells, corresponding to a specific activity of 100 units/mg.

Synonyms	apm-1, Adipolean (human)
Sequence	KGEPGEGAYV YRSAFSVGLE TYVTIPNMPI RFTKIFYNQQNHYDGSTGKF HCNIPGLYYF AYHITVYMKD VKVSLFKKDKAMLFTYDQYQ ENNVDQASGS VLLHLEVGDQ VWLQVYGEGERNGLYADNDN DSTFTGFLLY HDTN

Purity	95% by SDS-PAGE analysis.
Storage	Lyophilized recombinant human gAcrp30/Adipolean (rhgAcrp30) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhgAcrp30 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500258-1	IX03091-1	1mg	$1872		Add to Cart
BHP18500258-2	IX03091-10	10ug	$82		Add to Cart
BHP18500258-3	IX03091-50	50ug	$199		Add to Cart

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