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Home / Proteins & Peptide

G9a (EHMT2), GST-tag (E. coli-derived)

SKU : BHP18200400

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Mfr.No.	51000
Description	Human G9a, also known as EHMT2 (euchromatic histone-lysine N-methyltransferase 2) and KMT1C (GenBank Accession No. NM_006709), a.a. 785-1210, with N-terminal GST-tag, MW = 74.6 kDa, expressed in an E. coli expression system. Specific activity: 22 pmol/min/ug
Background	EHMT2 (G9a) is a lysine-preferring histone methyltransferase. EHMT2 can methylate itself at K165 and this automethylation is necessary and sufficient to mediate in vivo interaction of EHMT2 with heterochromatin protein-1 (HP1) (1). EHMT2 is responsible for the majority of dimethylation of histone H3 at lysine 9 (H3K9me2) and is required for the efficient repression of developmentally regulated genes during embryonic stem cell differentiation (2). EHMT2 is overexpressed in various cancers and can can specifically methylate p53 at Lys(373). EHMT2 polymorphisms have been associated with colorectal cancer.
Immunogen Region	785-1210
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 20 uM S-adenosylmethionine and 0-40 ng G9a) is added to wells coated with the substrate. Incubate for 1 hour. Add antibody against methylated K9 residue of histone H3, incubate at room temperature for 1 hour. Finally, incubate 1 hour with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (lambdaexc=330 nm, lambdaem=620 nm). Avoid freeze/thaw cycles.
GenBank	NM_006709
Mw(kda)	74.6 kDa

Synonyms	G9a, EHMT2, KMT1C, euchromatic histone-lysine N-methyltransferase 2
Uniprot	Q96KQ7

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Yuan,X. et al., Mol. Cell 27, 4, 585-595, 2007. 2. Nishida,M. et al., Genes Cells 12, 7, 877-888, 2007. Application Reference(s): On the histone lysine methyltransferase activity of fungal metabolite chaetocin (2013)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200400	51000	50 ug	$396		Add to Cart

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