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Home / Proteins & Peptide

G-CSF, Human

SKU : BHP18500181

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iXCells Biotechnologies

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Mfr.No.	IX02974, IX02974-1, IX02974-10, IX02974-50
Description	Granulocyte Colony-Stimulating Factor (G-CSF) contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17.Recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against 25mM Tris, pH8.0.
Molecular Weight	18.8kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Human
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	ED50 0.1ng/ml, measured by a cell proliferation assay of M-NFS-60 cells, corresponding to a specific activity of 1.0x 107 IU/mg.

Synonyms	CSF-3, C17 or f33,
Sequence	MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATYKLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLHSGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQQMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQSFLEVSYRVLR HLAQP

Purity	95% by SDS-PAGE and HPLC analyses.
Storage	Lyophilized recombinant human Granulocyte Colony-Stimulating Factor (rhG-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500181-1	IX02974-1	1mg	$2097		Add to Cart
BHP18500181-2	IX02974-10	10ug	$95		Add to Cart
BHP18500181-3	IX02974-50	50ug	$313		Add to Cart

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