Human FYN (GenBank Accession No. NM_002037), a.a 2-537(end) with N-terminal FLAG-tag, MW= 61 kDa, expressed in Sf9 insect cells.
Host cell line: Sf9 cells
Specific activity: 200 pmol/min/ug.
Immunogen Region
2-537(end)
Tag
N-terminal FLAG-tag
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, and 10% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 uM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 min. at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_002037
Mw(kda)
61 kDa
Synonyms
YES, SLK, Srk-Like Kinase
Uniprot
P06241
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Ban, K. et al., Blood, 111 (5), 2409-8 (2008) 2. Hoe, HS. et al., J.Biol.Chem 283 (10), 6288-99 (2008)