Human fucokinase (GenBank Accession No. NM_145059), a.a. 2-1084(end) with N-terminal FLAG-tag, MW = 118.6 kDa, expressed in baculovirus using an Sf9 insect cell expression system.
Specific activity: >=85.3 pmole/min/ug
Immunogen Region
2-1084(end)
Tag
N-terminal FLAG-tag
Formulation
40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, 20% glycerol, and 80 ng/ul FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The reaction mixture contained 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 2 mM L-Fucose, 100 uM ATP, and various amount of fucokinase in a final volume of 50 uL. The reaction was incubated at 37°C for 1 hr, and the amount of ATP consumed was calculated using Kinase-Glo Plus reagent (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_145059
Mw(kda)
118.6 kDa
Synonyms
fucokinase, FuK
Uniprot
Q8N0W3
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Hinderlich, S., et al., Biochem Biophys Res Commun. 2002 Jun 14; 294(3):650-4. 2. Zeitler, R., et al. J. Enz. Inhib. 1997 Feb; 11(4):265-73