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Home / Proteins & Peptide

FTO, His-Tag (Sf9 derived)

SKU : BHP18200763

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Mfr.No.	79480
Description	Human FTO, also known as Fat mass and obesity-associated protein, ALKBH9; BMIQ14; GDFD; KIAA1752, and alpha-ketoglutarate-dependent dioxygenase, GenBank Accession No. NM_001080432, a.a 32-505 (end), with N-terminal His-Tag and expressed in a Baculovirus infected Sf9 cell expression system. MW = 56 kDa. Specific activity: 0.0045 pmol/min/ug
Immunogen Region	32-505
Tag	N-Terminal His-Tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 180 mM Imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay done using ELISA technology. 50 ul reaction mix containing HEPES-based buffer and 0-1500 ng FTO is added to the wells coated with the substrate. Incubate overnight at RT. Add antibody against N6-methyladenosine, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminsecent substrates and read luminescence. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank	NM_001080432
Mw(kda)	56 kDa

Synonyms	Fat mass and obesity-associated protein, ALKBH9, BMIQ14, GDFD, KIAA1752, alpha-ketoglutarate-dependent dioxygenase
Uniprot	Q9C0B1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution.
Storage	At least 6 months at -80°C.

Reference	1. Frayling, T., et al., Science. 2007; 316(5826): 889-894.2. Dina, C., et al., Nature Genetics. 2007; 39(6): 724-726.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200763	79480	20 ug	$528		Add to Cart

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