Human Flt3 (GenBank Accession No. NM_004119), a.a. 564-end with N-terminal His-tag, MW=55 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 225 pmol/min/ug.
Immunogen Region
564-end
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 125 mM imidazole, 50% glycerol, and 3 mM DTT
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 uM of ATP. Substrate : 2 uM Tyr peptide 2 from Invitrogen.