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Home / Proteins & Peptide

Flt-3L, RhesusMacaque

SKU : BHP18500056

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iXCells Biotechnologies

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Mfr.No.	IX02762, IX02762-1, IX02762-10
Description	Flt-3 ligand (FL) is a recently identified hematopoietic cytokine whose activities are mediated by binding to the transmembrane glycoprotein Flt-3. Flt-3 was first discovered as a member of the class III subfamily of receptor tyrosine kinases (RTK) whose expression among hematopoietic cells was found to be restricted to highly enriched stem/progenitor cell populations. Additional class III RTKs include the receptors from SCF, M-CSF and PDGF. Not surprisingly, Flt-3 ligand is also structurally related to M-CSF and SCF. All three cytokines have been shown to exist both as type I transmembrane proteins and as soluble proteins.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <= -20 °C. Further dilutions should be made in appropriate buffered solutions.
Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Molecular Weight	Approximately 18.0 kDa, a single non-glycosylated polypeptide chain containing 159 amino acids.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Source	E. coli
Host	RhesusMacaque
Usage	This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE
Endotoxin Level	Less than 1 EU/ug of rRhFlt-3L as determined by LAL method.
Bioactivity	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human AML5 cells is less than 1.0 ng/ml, corresponding to a specific activity of 1.0 x 106 IU/mg.

Sequence	TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVPSNLQDEELCGALWRL VLAQRWMERL?KTVAGSKMQG LLERVNTEIH FVTKCAFQHPPSCLRFVQTN ISRLLQETSE QLVALKPWIT?RQNFSRCLEL QCQPDSSTLPPPRSPGALEA TALTAPQRP

Purity	97 % by SDS-PAGE and HPLC analyses.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500056-1	IX02762-1	1mg	$3775		Add to Cart
BHP18500056-2	IX02762-10	10ug	$220		Add to Cart

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