Human FGFR4 (GenBank Accession No. NM_002011), a.a. 460-end with N-terminal GST-tag, MW= 65 kDa, expressed in Sf9 cells via a baculovirus expression system
Host cell line: Sf9 cells
Specific activity: 50 pmol/min/ug
Immunogen Region
460-end
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM glutathione, 3 mM DTT, and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Reaction is conducted in a Kinase Buffer (50 mM Tris pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA and 1 mM DTT) containing 20 uM ATP and 0.2 mg/ml poly-(Glu-Tyr) for 30 minutes at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega).