Human Fibroblast growth factor receptor 3 (FGFR3), also known as JTK4, CD333, CEK2 and ACH, GenBank Accession No. NM_00142, a.a. 447-761, with N-terminal His-Avi-tag, expressed in an Sf9 infected baculovirus expression system and enzymatically biotinylated using Avi-tag technology. Biotinylation is confirmed to be>=90%. MW = 39 kDa. This protein was isolated from a co-expression with PTP1B. Percentage of phosphorylation<=5%.
Specific activity: 4.3 pmole/min/ug
Immunogen Region
447-761
Tag
N-terminal His-Avi-tag
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done in Kinase buffer containing 2 mM DTT using 0.2 mg/mL Poly-(Glu:Tyr) substrate and 10 uM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_00142
Mw(kda)
39 kDa
Synonyms
JTK4, CD333, CEK2, ACH
Uniprot
P22607
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Cappellen, D. et al., Nature Genetics. (1999); 23:18-20. 2. Bellus, G.A. et al., AM. J. Hum. Genet. (1995); 56(2):368-373.