Dephosphorylated recombinant FGFR3 (Fibroblast Growth Factor Receptor 3) encompassing amino acids 397-806. This protein contains an N-terminal His-Tag and has been biotinylated using an N-terminal Avi-Tag. The recombinant protein is purified to>=90%
Specific activity: 4.3 pmole/min/ug
Immunogen Region
447-761
Tag
N-terminal His-Avi-tag
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT.
Species
Human
Notes
Assay was done in Kinase buffer containing 2 mM DTT using 0.2 mg/mL Poly-(Glu:Tyr) substrate and 10 uM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_000142
Mw(kda)
47 kDa
Synonyms
JTK4, CD333, CEK2, ACH
Uniprot
P22607
Shipping Temperature
-80°C (dry ice)
Purity
>=90%
Storage
At least 6 months at -80°C.
Reference
1. Cappellen, D. et al., Nature Genetics. (1999); 23:18-20. 2. Bellus, G.A. et al., AM. J. Hum. Genet. (1995); 56(2):368-373.