Human FGFR2 (GenBank Accession No. NM_000141), a.a. 399-end with N-terminal GST-tag, MW= 74 kDa, expressed in a baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: 521.4 pmol/min/ug.
Immunogen Region
399-end
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 uM of ATP. Substrate: 2 uM Tyr peptide 4 from Invitrogen.