Human Fibroblast growth factor receptor 1 (FGFR1), also known as BFGFR, CD331, CEK and FLT2, GenBank Accession No. NM_0023110.3, a.a. 399-822(end), with C-terminal Avi-His-tag, co-expressed with PTP1B for dephosphorylation in an Sf9 cell expression system. Percentage of phosphorylation<=5% and enzymatically biotinylated using Avi-tag technology. Biotinylation is confirmed to be>=90%. MW=51 kDa.
Immunogen Region
399-822(end)
Tag
C-terminal Avi-His-Tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.02% Tween, 20% glycerol, 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done according to TYR Phosphorylation Assay Kit (Biohippo #79502) with FGFR enzyme titration range 0 ng/rxn-500 ng/rxn.