Human feline sarcoma oncogene (FES), GenBank Accession # NM_002005, a.a. 2-822 (end), N-terminal His-tag and N-terminal FLAG-tag, MW=95.3 kDa, expressed in Sf9 insect cells via a baculovirus expression system
Host cell line: Sf9 cells
Specific activity: 235 pmol/min/ug
Immunogen Region
full length
Tag
N-terminal His-FLAG-tags
Formulation
40 mM Tris-HCl, pH 7.5, 110 mM NaCl, 3 mM DTT, 2.2 mM KCl, 20% glycerol, 80 ug/ml FLAG peptide.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was performed in a Kinase buffer (40 mM Tris pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA) containing 1 mM DTT using Poly-(Glu4:Tyr)substrate (0.2 mg/ml) and 20 uM ATP for 30 minutes at 30°C. The amount of ATP transferred was alculated using Kinase-Glo Plus reagent (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_002005
Mw(kda)
125 kDa
Synonyms
feline sarcoma oncogene, FES
Uniprot
P07332
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Hellwig S, Smithgall TE. Front Biosci. 2011 Jun 1,17:3146-55. 2. Kanda S, Miyata Y. Front Biosci. 2011 Jan 1,16:1024-35.