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Home / Proteins & Peptide

FDH, P. putida, His-tag

SKU : BHP18200563

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Mfr.No.	71076
Description	Pseudomonas putida formaldehyde dehydrogenase (FDH) a.a. 2 - 399 (end) (GenBank Accession No. D21201) with N-terminal His-tag, MW = 42.9 kDa, expressed in an E. coli expression system. Specific activity: >=35 umol/min/ug
Immunogen Region	2 - 399 (end)
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Species	Pseudomonas putida
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The reaction was monitored by measuring NADH production by following absorbance at 340nm at room temperature for 5min. Buffer: pH 7.3, 20 mM Tris, 50 mM NaCl, 20 uM Fe+2, 500 mM a-ketoglutarate, 2 mM ascorbic acid, 0.01% Tween-20, and 1 mM NAD+. Avoid freeze/thaw cycles.
GenBank	D21201
Mw(kda)	42.9 kDa

Synonyms	formaldehyde dehydrogenase, FDH
Uniprot	P46154

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Ito, K., et al., J Bacteriol. 1994;176:2483-2491. 2. Tanaka, K., et al., J Mol Biol. 2002;324:519-533.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200563	71076	100 ug	$407		Add to Cart

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