Mouse FcRn also known as Neonatal Fc receptor is a heterodimer of FCGRT (Fc Fragment of IgG Receptor and Transporter) and B2M (beta 2 microglobulin), GenBank Accession numbers NM_010189 and NM_009735; a.a. 22-297 and a.a. 1-119 respectively. FCGRT has a C-terminal His-Avi-tag. This protein is enzymatically biotinylated using AviTagTM technology. These proteins are co-expressed in a HEK293 expression system. FcRn MW = 34 kDa, B2M MW = 14 kDa.
Immunogen Region
FcRn: 22-297, B2M: 1-119
Tag
C-terminal His-Avi-Tag
Formulation
8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species
Human
Application
Useful for the study of FcRn and Fc interactions and binding kinetics.
Notes
Avoid freeze/thaw cycles.
GenBank
FcRn: NM_010189, B2M: NM_009735
Mw(kda)
FcRn: 34 kDa, B2M: 14 kDa
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary use carrier protein (BSA 0.1 - 0.5%).
Reference
1. Roopenian, D., et al., Nature. 2007 Sep 7: 715-7252. Shields, R. et al., Biological Chemistry. 2000 Nov; 276:6591-6604.