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Home / Proteins & Peptide

FBXL10 (KDM2B, JHDM1B), FLAG-tag

SKU : BHP18200344

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BPS Bioscience

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Mfr.No.	50120
Description	Human F-Box Protein 10 (FBXL10, also known as KDM2B, JHDM1B, CXXC2, and Histone-H3 K36 demethylase 1B), GenBank Accession No. NM_032590, a.a. 1-650, with C-terminal FLAG-tag, MW= 75 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells
Immunogen Region	1-650
Tag	C-terminal FLAG-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme wasincubated with 0.4 uM substrate inHEPES-containing JMJ assay buffer SAand SA- (without KG) in a 10-ul reactionfor 180 min at 30°C. 5 ul of Anti-RabbitAlphaLISA acceptor beads (1:250) and 5ul of rabbit polyclonal antibody (1:200)were added and incubation continued for30 min. Streptavidin Donor beads (1:125)were added (10 ul) and mixture was furtherincubated for 10 min at RT before Acountswere measured. Avoid freeze/thaw cycles.
GenBank	NM_032590
Mw(kda)	75 kDa

Synonyms	F-Box Protein 10, FBXL10, KDM2B, JHDM1B, CXXC2, Histone-H3 K36 demethylase 1B
Uniprot	Q8NHM5

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=83% by HPLC
Storage	At least 6 months at -80°C.

Reference	1. Tanaka, Y., et al. (2010). EMBO J. 29(9):1510-22. 2. Blackledge, N.P., et al. (2010). Mol. Cell. 38(2):179-90. 3. Frescas, D., et al. (2008). Cell Cycle 7(22):3539-47.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200344	50120	20 ug	$522.50		Add to Cart

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