Human FAK (GenBank Accession No. L13616), a.a. 411-686 with N-terminal GST-tag, MW=62 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 100 U/mg.
Unit definition: One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30°C.
Immunogen Region
411-686
Tag
N-terminal GST-tag
Formulation
25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na orthovanadate, 0.1 mM ATP, 30ug/ml Poly (Glu:Tyr)4:1 substrate, and 1 ug/ml recombinant FAK.
Avoid freeze/thaw cycles.
GenBank
L13616
Mw(kda)
62 kDa
Synonyms
FAK, focal adhesion kinase
Uniprot
Q05397
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Lim, ST. et al., Mol Cell. 2008 Jan 18,29(1):9-22. 2. Gabarra-Niecko, V. et al., Cancer Metastasis Rev. 2003 Dec,22(4):359-74.