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Home / Proteins & Peptide

EZH2/EED/SUZ12/RbAp48, His-tag, FLAG-tag

SKU : BHP18201813

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Mfr.No.	51099
Description	Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end with N-terminal His-tag, MW = 87 kDa, and human RbAp48 (NM_005610), a.a. 2-end with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system. host species: insect Host species: insect Host cell line: Sf9 Specific activity: 0.046 pmol/min/ug
Background	EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Immunogen Region	2-end (all components)
Tag	N-terminal His-tag (SUZ12, EZH2, RbAP48), N-terminal FLAG-tag (EED)
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, and 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 20 uM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hour. Add antibody against methylated K27 residue of histone H3, incubate 1 hour. Then incubate 1 hour with the HRP-labeled secondary antibody, followed by addition of the HRP substrate to produce chemiluminescence. Avoid freeze/thaw cycles.
GenBank	NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_005610(RbAP48)
Mw(kda)	272 kDa (complex)

Synonyms	KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2; RBAP48, Retinoblastoma Binding Protein 4 (RBBP4), NURF55, Chromatin Assembly Factor 1 Subunit C, CAF1 p48; Embryonic Ectoderm Development, hEED, EED, WAIT-1; SUZ12, Suppressor Of Zeste 12 Homolog, CHET9, JJA
Uniprot	Q15910

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Varambally, S. et al. Science 322 (5908), 1695-1699 (2008). 2. Rakotobe, D. et al., Virol. J. 5, 32 (2008).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201813	51099	50 ug	$902		Add to Cart

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