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Home / Proteins & Peptide

EZH2/EED/SUZ12/RbAp48/AEBP2 Complex, FLAG-tag, His-tag

SKU : BHP18200404

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Mfr.No.	51004
Description	Complex of human EZH2 (GenBank Accession No. NM_004456), a.a. 2-end, with N-terminal His-tag, MW= 86 kDa, human EED (NM_003797), a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12 (NM_015355), a.a. 2-end, with N-terminal His-tag, MW = 87 kDa, Human AEBP2 (NM_153207), a.a. 2-end, with N-terminal His-tag, MW= 53 kDa, and human RbAp48 (NM_005610), a.a. 2-end, with N-terminal His-tag, MW = 48 kDa, co-expressed in a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.08 pmol/min/ug
Background	EZH2 is the catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys9 (H3K9me) and Lys27 (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4.
Immunogen Region	2-end (all components)
Tag	N-terminal FLAG-tag(EED), N-terminal His-tag (other components)
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (20 mM phosphate buffer pH 7.4, 0.05% Tween-20, 20 uM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence. Avoid freeze/thaw cycles.
GenBank	NM_004456 (EZH2), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
Mw(kda)	325 kDa (complex)

Synonyms	KTM6A, EZH2, PRC2, Enhancer Of Zeste Homolog 2; RBAP48, Retinoblastoma Binding Protein 4 (RBBP4), NURF55, Chromatin Assembly Factor 1 Subunit C, CAF1 p48; Embryonic Ectoderm Development, hEED, EED, WAIT-1; SUZ12, Suppressor Of Zeste 12 Homolog, CHET9, JJA
Uniprot	Q15910

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Morin, R., et al. (2010). Nature Genetics 42, 181 - 185. 2. 2. Varambally, S. et al. (2008). Science 322 (5908), 1695-1699. 3. Rakotobe, D. et al. (2008). Virol. J. 5, 32. Application Reference(s): 1. Arcand, M., et al., ""Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases."" Poster presented as part of the BioTechniques Fall 2011 Epigenetics Meeting. 2. Yap, D.B, et al., "Somatic mutations at EZH2 Y641 act dominantly through a mechanism of selectively altered PRC2 catalytic activity, to increase H3K27 trimethlyation." (2010). Blood. 117: 2451-2459.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200404	51004	50 ug	$995.50		Add to Cart

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