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Home / Proteins & Peptide

EPHB4, His-tag

SKU : BHP18200190

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Mfr.No.	40201
Description	Human EPHB4 (GenBank Accession No. NM_004444), a.a. 563-987 with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 43 pmole/min/ug Unit definition: One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30°C.
Immunogen Region	563-987
Tag	N-terminal His-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	ADP-Glo assay with 0.2 mg/ml poly-(Glu4:Tyr) as a substrate and 100 uM ATP at final concentration. Reaction was done for 50 minutes at 30°C. ADP-Glo for 45 minutes at RT, and Kinase detection for 55 minutes at RT. Avoid freeze/thaw cycles.
GenBank	NM_004444
Mw(kda)	50 kDa

Synonyms	EphB4, Ephrin Type-B Receptor 4, Hepatoma Transmembrane Kinase, HTK, Tyro11, MYK1
Uniprot	P54760

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	>12 months at -80°C

Reference	1. Bruhl,T., et al., Circ. Res. 94 (6), 743-751 (2004). 2. Fuller,T., et al., J. Cell. Sci. 116 (Pt 12), 2461-2470 (2003).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200190	40201	10 ug	$390.50		Add to Cart

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