Human EPHB2 (GenBank Accession No. NM_004442), a.a. 569-end with N-terminal His-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 21.8 pmol/min/ug.
Immunogen Region
569-end
Tag
N-terminal His-tag
Formulation
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35 and 2 uM substrate (Invitrogen Zlyte Peptide 1) at room temperature for 1 hour.