Human EPHA2 (GenBank Accession No. NM_004431), a.a. 596-end with N-terminal His-tag, MW=47 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 170 U/mg.
Unit definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of tyrosine substrate per minute at pH 7.4 and 30°C.
Immunogen Region
596-end
Tag
N-terminal His-tag
Formulation
50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol and 3.25 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 M Na orthovanadate, 0.1 mM ATP, 30 g/ml Poly (Glu:Tyr) 4:1 substrate, and 0.1 g /ml recombinant EPHA2.
Avoid freeze/thaw cycles.
GenBank
NM_004431
Mw(kda)
47 kDa
Synonyms
EphA2, Ephrin Type-A Receptor 2, CTPP1, ECK
Uniprot
P29317
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
>12 months at -80°C
Reference
1. Nakada,M., et al., Cancer Res. 64 (9), 3179-3185 (2004). 2. Kataoka,H.,et al., J. Cancer Res. Clin. Oncol. 128 (7), 343-348 (2002).