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Home / Proteins & Peptide

Enterokinase, His, Bovine

SKU : BHP18500207

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Mfr.No.	IX03004, IX03004-100, IX03004-500, IX03004-5000
Description	Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys. Enterokinase will not work if the recognition site is followed by a proline. rbEK with 6 x His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.?Recombinant Bovine Enterokinase (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids. A fully biologically active molecule, rbEK has a molecular mass of 22.7 kDa and is obtained by proprietary chromatographic techniques. Components: 100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl 2, 50% glycerol)
Features	Components: 100 IU(or 500IU or 5000IU) Recombinant Bovine Enterokinase (in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol) ?100ug Cleavage Control Protein (Lyophilized after extensive dialysis against PBS, pH 7.0) ?3.6 ml EK Dilution/Storage Buffer (20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2, 50% glycerol)?1.8 ml 10X EK Cleavage/Capture Buffer (200mM Tris-HCl, pH 7.4, 500mM NaCl, 20mM CaCl2)?Storage: Store kit components at 20°C
Formulation	Sterile liquid solution contians 20mM Tris, 200mM NaCl, 2mM CaCl2, 50% glycerol, pH 7.4.
Molecular Weight	Theoretical MW: 22.7 kDa.Apparent MW: 40.0 kDa, observed by reducing SDS-PAGE.
Source	P. pastoris
Host	Bovine
Endotoxin Level	1.0 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	5 IU/ul.Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 ug of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.

Synonyms	Enteropeptidase, ENTK, PRSS7

Purity	95% by SDS-PAGE analyses.
Storage	Recombinant Bovine Enterokinase (rbEK) remains stable up to 1 year at -20°C from date of receipt. It will remain stable at 37°C for one week without losing any activity.Please avoid freeze-thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500207-1	IX03004-100	100IU	$62		Add to Cart
BHP18500207-2	IX03004-500	500IU	$157		Add to Cart
BHP18500207-3	IX03004-5000	5000IU	$968		Add to Cart

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