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Home / Proteins & Peptide

Enterokinase (EK), His, Lyophilized, Bovine

SKU : BHP18500494

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iXCells Biotechnologies

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Mfr.No.	IX03376, IX03376-1, IX03376-10, IX03376-50
Reconstitution	Reconstitute lyophilized EK powder in sterile deionized water at 1mg/ml, then dilute in EK Dilution/Storage Buffer according to your requirement.
Description	Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK?). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used wildly in biochemical applications. rbEK with 6 x His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system.?Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 x His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by proprietary chromatographic techniques.
Formulation	Lyophilized from a 0.2 um filtered solution in 20mM Tris-HCl, pH 7.4, 200mM NaCl, 2mM CaCl2.
Molecular Weight	40 kDa, observed by reducing SDS-PAGE.
Source	P. pastoris
Host	Bovine
Endotoxin Level	0.2 EU/ug, determined by LAL method.
Notes	For research use only
Bioactivity	100 IU/ug

Synonyms	Enteropeptidase, ENTK, PRSS7

Purity	95% as analyzed by SDS-PAGE.
Storage	Lyophilized recombinant Bovine Enterokinase (His-tagged) (rbEK) remains stable up to one year at -20°C from date of receipt. Please avoid freeze-thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500494-1	IX03376-1	1mg	$844		Add to Cart
BHP18500494-2	IX03376-10	10ug	$75		Add to Cart
BHP18500494-3	IX03376-50	50ug	$113		Add to Cart

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