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Home / Proteins & Peptide

ELANE (Elastase), Avi-His-Tag, Biotin-Labeled HiP

SKU : BHP18201385

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BPS Bioscience

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Mfr.No.	101142-1
Description	Recombinant human Elane (Neutrophil Elastase), encompassing amino acids 28-252. The construct contains a C-terminal Avi-Tag followed by a His-tag (6xHis). The recombinant protein was enzymatically biotinylated using the Avi-Tag and affinity purified. HiP indicates a high purity protein (>=90% pure) and less than 10% aggregation as measured by gel filtration. The protein appears as two separate bands in SDS PAGE due to slightly different glycosylation patterns. The identity of the protein has been confirmed by protein sequencing (by Edman degradation) of the N-terminus. Both bands are recognized by an anti-His antibody, indicating that the C-terminus is intact. Constructs: ELANE (28-252-Avi-His)-(Biotin)
Immunogen Region	28-252
Tag	C-terminal Avi-His-tag
Formulation	8 mM phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl, 20% glycerol
Species	Human
Notes	Avoid freeze/thaw cycles.
GenBank	NM_001972
Mw(kda)	27 kDa + glycans

Synonyms	Elastase, Elastase-2, ELA2, Human leukocyte elastase, HLE, Medullasin, PMN elastase, PMN-E, GE, NE, HNE, SCN1
Uniprot	P08246

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=90%
Storage	At least 6 months at -80°C.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201385	101142-1	25 ug	$324.50		Add to Cart

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