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Home / Proteins & Peptide

EGFR (T790M, L858R), GST-tag

SKU : BHP18200227

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BPS Bioscience

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Mfr.No.	40350
Description	Human EGFR T790M L858R (GenBank Accession No. NM_005228), a.a. 668-1210 with substitutions of Threonine790 to Methionine and Leucine858 to Arginine and an N-terminal GST-tag, MW= 88 kDa, expressed in a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 1,200 pmol/min/ug.
Immunogen Region	668-1210
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 8.5, 250 mM NaCl, 10% glycerol and 2 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 mM ATP. Reaction was done at 30°C for 30 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_005228
Mw(kda)	88 kDa

Synonyms	EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
Uniprot	P00533

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Adachi,S. et al., Cancer Res. 67 (13), 6493-6501 (2007) 2. Rosano,L. et al., Cancer Res. 67 (13), 6351-6359 (2007). Application Reference: Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200227	40350	10 ug	$390.50		Add to Cart

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