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Home / Proteins & Peptide

EGFR (T790M), GST-tag

SKU : BHP18200184

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BPS Bioscience

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Mfr.No.	40188
Description	Human EGFR T790M (GenBank Accession No. NM_005228), a.a. 668-1210 with substitution of Threonine790 to Methionine and an N-terminal GST-tag, MW= 88 kDa, expressed in a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 113 pmol/min/ug.
Immunogen Region	668-1210
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	The enzyme reaction was carried out for 1 hour at room temperature in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EDTA, 0.01% BRIJ-35 and 200 uM of ATP. Substrate : 2 uM Tyr peptide 4 from Invitrogen. Avoid freeze/thaw cycles.
GenBank	NM_005228
Mw(kda)	88 kDa

Synonyms	EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
Uniprot	P00533

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Adachi,S. et al., Cancer Res. 67 (13), 6493-6501 (2007) 2. Rosano,L. et al., Cancer Res. 67 (13), 6351-6359 (2007). Application Reference: Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200184	40188	10 ug	$390.50		Add to Cart

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