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Home / Proteins & Peptide

EGFR (T790M, C797S, L858R), GST-tag

SKU : BHP18200228

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BPS Bioscience

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Mfr.No.	40351
Description	Human EGFR T790M,C797S, L858R (GenBank Accession No.NM_005228), (a.a. 668-1210) with NterminalGST tag, MW = 88 kDa,expressed in baculovirus expressionsystem. Host cell line: Sf9 cells Specific activity: 150 pmol/min/ug
Immunogen Region	668-1210
Tag	N-terminal GST tag
Formulation	40 mM Tris pH 8.0, 110 mMNaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mMDTT and 20% glycerol
Mutation	T790M, C797S, L858R
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was done in Kinase buffer containing 0.2 mM DTT using Poly- (Glu4:Tyr) substrate (0.2 mg/ml) and 20 uM ATP. Reaction was done at 30°C for 40 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank	NM_005228
Mw(kda)	88 kDa

Synonyms	EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
Uniprot	P00533

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Thress, K.S., et al., Nat. Med. 2015; 21:560-562. 2. Niederst, M.J., et al., Clin. Canc. Res. 2015.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200228	40351	10 ug	$401.50		Add to Cart

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