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Home / Proteins & Peptide

EGFR, GST-tag

SKU : BHP18200183

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BPS Bioscience

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Mfr.No.	40187
Description	Human EGFR (GenBank Accession No. NM_005228), a.a. 668-1210 with N-terminal GST-tag, MW=87.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 800 pmol/min/ug.
Immunogen Region	668 -1210
Tag	N-terminal GST-tag
Formulation	50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol and 1 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Assay was performed in a kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 uM ATP. Reaction was done at 30°C for 30 min. ATP transferred was calculated using Kinase-Glo reagent (Promega). Avoid freeze/thaw cycles.
GenBank	NM_005228
Mw(kda)	87.3 kDa

Synonyms	EGFR, Epidermal Growth Factor Receptor, ERBB, ErbB-1, HER1, MENA
Uniprot	P00533

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Purity	>=70%
Storage	At least 6 months at -80°C.

Reference	1. Nakamura, J.L. Expert Opin. Ther. Targets 11 (4), 463-472 (2007). 2. Kim, H.J., et al. Curr. Biol. 17 (5), 455-461 (2007). Application Reference: Figueroa, M., et al., J. Antibiotics. 65, 559-564 (2012).Product Specific Reference(s): 1. Mowafy, S., et al., Eur J Med Chem. 61, 132-45 (2013).

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200183	40187	10 ug	$434.50		Add to Cart

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