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Home / Proteins & Peptide

EGF, Human

SKU : BHP18500025

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iXCells Biotechnologies

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Mfr.No.	IX02691, IX02691-1, IX02691-100, IX02691-500
Description	Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.?Recombinant human Epidermal Growth Factor (rhEGF) produced in E. coli is a non-glycosylated polypeptide chain of 54 amino acids. A fully biologically active molecule, rhEGF has a molecular mass of 6.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques.
Reconstitution	Reconstituted in ddH2O at 100 ug/ml.
Formulation	Lyophilized after extensive dialysis against 10 mM PB, pH 7.0.
Molecular Weight	6.2kDa, observed by reducing SDS-PAGE.
Source	E. coli
Host	Human
Endotoxin Level	Less than 0.1 ng/ug (1 EU/ug) determined by LAL test
Bioactivity	ED50 0.6ng/ml, measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts in serum-free medium and CellTiter-Glo cell viability assay, corresponding to a specific activity of 1.7 x 106 units/mg.

Synonyms	Urogastrone, URG
Sequence	MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIGERCQYRDLKW WELR

Purity	95% by SDS-PAGE analysis.
Storage	Lyophilized recombinant human Epidermal Growth Factor (rhEGF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500025-1	IX02691-1	1mg	$107		Add to Cart
BHP18500025-2	IX02691-100	100ug	$49		Add to Cart
BHP18500025-3	IX02691-500	500ug	$95		Add to Cart

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