Human Epidermal Growth Factor (GenBank Accession No. NM_001963), a.a. 971- 1023, MW=6.2 kDa, expressed in an E. coli expression system.
Reconstitution
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Immunogen Region
971- 1023
Tag
none
Formulation
Lyophilized from a sterile filtered solution containing 10 mM Phosphate Buffered Saline (PBS), pH 7.4, 138 mM NaCl, and 2.7 mM KCl.
Species
Human
Application
Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Endotoxin Level
< 1EU per ug of EGF as determined by the LAL method.
Notes
Avoid freeze/thaw cycles.
Biological Activity Comment
ED50 is< 0.1 ng/ml. ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells
GenBank
NM_001963
Mw(kda)
6.2 kDa
Synonyms
epidermal growth factor, EGF, beta-urogastrone
Uniprot
P01133
Shipping Temperature
-20°C or -80°C
Format
lyophilized protein
Purity
>=98% by SDS-PAGE and HPLC analysis.
Storage
>=2 years (lyophilized) at -20°C.
Reference
1. Su, X., et al. J. Biol. Chem. 282 (29),21278-21284 (2007) 2. Jin, G., et al. Cancer Sci. 98 (6), 864-868 (2007)