Human Embryonic Ectoderm Development (hEED), also known as WAIT-1, GenBank Accession No. NM_003797, a.a. 2-end with N-terminal FLAG-tag, MW= 51 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Immunogen Region
2-end
Tag
N-terminal FLAG-tag
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 90 ug/ml FLAG peptide, and 10% glycerol.
Species
Human
Application
Useful for binding studies to investigate protein-protein interactions in the EZH2 complex. Also suitable as an immunogen or as a standard for Western blotting.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_003797
Mw(kda)
51 kDa
Synonyms
Embryonic Ectoderm Development, hEED, EED, WAIT-1
Uniprot
O75530
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Schumacher, A., et al. (1998) Genomics 54 (1): 79-88. 2. van der Vlag, J., et al. (1999). Nat. Genet. 23 (4): 474-8.