Human Dual Specificity Tyrosine Phosphorylation Regulated Kinase 1B (DYRK1B), also known as Minibrain-Related Kinase (MIRK), GenBank Accession No. NM_004714, a.a. 2-629(end), with N-terminal GST-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW= 95 kDa.
Specific activity: >=500 pmole/min/ug
Immunogen Region
2-629(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0. 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% Glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done in Kinase buffer containing DYRKtide substrate peptide 1 (0.2 mg/ml) and 20 uM ATP. Reaction was done at 30°C for 35 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega).
Avoid freeze/thaw cycles.
GenBank
NM_004714
Mw(kda)
95 kDa
Synonyms
Minibrain-Related Kinase, MRK
Uniprot
Q9Y463
Shipping Temperature
-80°C (dry ice)
Storage
At least 6 months at -80°C.
Reference
1. Mao, J. et al., JBC. 277(38): 35156-35161, 2002. 2. Guimera, J. W. et al., Genomics. 57(3): 407-418, 1999.