Human DRAK2, (GenBank Accession No. NM_004226.3), a.a. 2-372 (end), with N-terminal GST-tag, MW = 69 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Immunogen Region
2-372 (end)
Tag
N-terminal GST-Tag
Formulation
50 mM HEPES, pH 7.5, 0.001% Brij-35, 1 mM DTT, 10 mM MgCl2, 150 mM KCl, 0.1 mM EDTA and 5% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done in Kinase buffer containing 1 mM DTT using MRCL3 peptide substrate (0.2 mg/ml) and 20uM ATP. Reaction was done at 30°C for 50 min.Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Avoid freeze/thaw cycles.
GenBank
NM_004226.3
Mw(kda)
69 kDa
Synonyms
STK17B, DAP kinase-related apoptosis-inducing protein kinase 2 or death-associated protein kinase-related 2
Uniprot
O94768
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1-0.5%).
Reference
1. McGargill, M. et al, Immunity. 21 (6): 781-791 (2004).2. Mao, J. et al, Journal of Biological Chemistry. 281: 12587-12595 (2006).